Cluster of Differentiation 112 (CD112), also known as poliovirus receptor-related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus and thus is involved in cell to cell spreading of these viruses. CD8+ T cells can kill infected B cell lines via the interaction between CD226/CD112 protein and DNAM-1 (CD226), suggesting that NK cell- and CD8+ T cell-mediated toxicity and cytokine production might be induced by the combination of these proteins. Cytoxan-CD112 has been recognized as a key element in allergic responses and may be considered a new target for anti-allergic medication. Human CD112/Nectin-2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human CD112/Nectin-2 Recombinant Protein (RPES2943)
Measured by its binding ability in a functional ELISA.Immobilized human CD112 at 10 µg/ml (100 ?l/well) can bind human DNAM1-Fch with a linear range of 1.25-10 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of recombinant human CD112 (NP_002847.1) extracellular domain (Met 1-Leu 360) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
