Interleukin-2 receptor (IL-2R), also known as high affinity IL-2 receptor subunit beta, Interleukin 2 receptor beta subunit, and IL-2RB, is a protein that plays an important role in T cell-mediated immunity. 3 variants of CD122/IL-2RB are present in terms of their capacity to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and does not participate in signal transduction. The alpha/beta subunit heterodimer forms the intermediate affinity form, whereas the alpha/beta/gamma subunit heterotrimer makes up the high affinity type. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The expression of CD122/IL-2RB was limited to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously in the absence of any stromal cells, but not to IL-15. This receptor has a high affinity form that is expressed on activated T lymphocytes, activated B cells, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development. Human CD122/IL-2RB Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human CD122/IL-2RB Recombinant Protein (RPES1400)
Product Code:
RPES1400
Size:
5µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD122,IL15RB,P70-75
Accession:
NP_000869.1
Sequence:
Met 1-Asp 239
Fusion tag:
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized human IL2Rb at 10 µg/ml (100 ?l/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 µg/mL.2. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1-4µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human IL2 R? (NP_000869.1) extracellular domain (Met 1-Asp 239) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
25.2 kDa
AP Mol Mass:
40 kDa
Formulation:
Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
