Poliovirus Receptor (PVR) is a 70 kDa type I transmembrane single-span glycoprotein that belongs to the nectin-like (Necl) family and was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV); an etiologic agent of the central nervous system disease poliomyelitis. PVR contains three Ig-like extracellular domains; a transmembrane segment; and a cytoplasmic tail. The normal cellular function of PVR maybe the involvement of intercellular adhension between epithelial cells. Alternate splicing of the PVR mRNA yields four different isoforms (?; β; ?; and ?) with identical extracellular domains.
Product Name:
Human CD155/PVR/NECL5 Recombinant Protein (RPES0478)
Product Code:
RPES0478
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Poliovirus Receptor, Nectin-Like Protein 5, NECL-5, CD155, PVR, PVS
Accession:
P15151
Sequence:
Trp21-Asn343(Ile340Met)
Fusion tag:
C-His
Activity:
Immobilized Human TIGIT-Fc(Cat: PKSH033510) at 5µg/ml(100 ?l/well) can bind Human PVR-His. The ED50 of Human PVR-His is 11.66 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human NECL5 is produced by our Mammalian expression system and the target gene encoding Trp21-Asn343(Ile340Met) is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
36.13 kDa
AP Mol Mass:
58 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
