Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.
Product Name:
Human CGREF1/CGR11 Recombinant Protein (RPES0452)
Product Code:
RPES0452
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Cell Growth Regulator with EF Hand Domain Protein 1, Cell Growth Regulatory Gene 11 Protein, Hydrophobestin, CGREF1, CGR11
Accession:
Q99674
Sequence:
Ala20-Ile301
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Cell Growth Regulator with EF Hand Domain Protein 1 is produced by our Mammalian expression system and the target gene encoding Ala20-Ile301 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
30.9 kDa
AP Mol Mass:
17-36 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,1mM GaCl2,pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
