C5a is a protein fragment released from complement component C5. During the classic, alternative, and lectin pathways of complement activation, C5a convertase on the C5 alpha-chain cleaves C5a to generate C5a in humans. C5a has a core region made up of four anti-parallel alpha-helices joined by three disulfide bonds, as well as a structured C-terminal tail. Carboxypeptidase B cleaves C5a to a 73-amino acid low activity form, C5a des-Arg. C5a is a highly inflammatory mediator, as well as a strong chemotactic attractant for neutrophils and other leukocytes. It increases histamine production, promotes permeability in the blood vessels, and increases the levels of several cytokines from leukocytes. It promotes neutrophil-endothelial cell adhesion, enhances the humoral and cell-mediated immune response. Carboxypeptidases act on C5a, converting it to the less active C5adesArg. C5a and C5adesArg stimulate the innate immune response by acting through a classical G protein-coupled receptor, CD88. Their actions at the non-G protein-coupled receptor C5L2 (or GPR77) are still unknown. C5a has a wide range of activities since it is widely expressed throughout the body. C5a has been identified as a key pathogenic player in several immuno-inflammatory diseases, making C5a inhibition a viable therapeutic approach. C5a is a powerful chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T cells, phagocytic cell inactivation and granule-based enzyme release, as well as the generation of oxidants, all of which may contribute to innate C5a anaphylatoxin is linked to a variety of ailments including rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis. Human Complement C5a Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Complement C5a Recombinant Protein (RPES1795)
Measured by its ability to induce N-acetyl-?-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is typically 5-15 ng/ml.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the active form of human C5a (Leu 679-Arg 751) was expressed.
Purity:
> 94 % as determined by reducing SDS-PAGE.
Mol Mass:
8.3 kDa
AP Mol Mass:
8.3 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
