DCAMKL1; also known as DCLK1; is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains; which bind microtubules and regulate microtubule polymerization; a C-terminal serine/threonine protein kinase domain; which shows substantial homology to Ca2+/calmodulin-dependent protein kinase; and a serine/proline-rich domain in between the doublecortin and the protein kinase domains; which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes; including neuronal migration; retrograde transport; neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.
Product Name:
Human DCAMKL1 Recombinant Protein (RPES1198)
Product Code:
RPES1198
Size:
50 µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
CL1,CLICK1,DCAMKL1,DCDC3A,DCLK
Accession:
O15075-1
Sequence:
Met 1-Val 705
Fusion tag:
N-His & GST
Activity:
The specific activity was determined to be 6.1 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
