As a member of the epidermal growth factor receptor (EGFR) family; EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF; amphiregulin; TGF-α; betacellulin; etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation; survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization; the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK; PI3K/PKB and STAT). In addition; EGFR signaling also has been shown to exert action on carcinogenesis and disease progression; and thus EGFR protein is proposed as a target for cancer therapy currently.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human EGFR/ErbB1 Recombinant Protein (RPES2136)
Product Code:
RPES2136
Size:
50 µg
Species:
Human
Expressed Host:
Baculovirus-Insect cells
Synonyms:
ERBB,ERBB1,HER1,mENA,NISBD2,PIG61
Accession:
NP_005219
Sequence:
Met 668-Ala 1210
Fusion tag:
N-His-GST
Activity:
The specific activity was determined to be 105 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
89.1 kDa
Formulation:
Supplied as sterile 20 mM Tris; 500 mM NaCl; 10 % glycerol; pH 7.4
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
