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Human DKK1/Dkk Recombinant Protein (RPES0094)

SKU:
RPES0094
Product Type:
Recombinant Protein
Species:
Human
€538
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Description

system_update_altDatasheet

Human DKK1/Dkk Recombinant Protein

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Product Name:Human DKK1/Dkk Recombinant Protein (RPES0094)
Product Code:RPES0094
Size:20µg
Species:Human
Expressed Host:HEK293 Cells
Synonyms:Dickkopf-related protein 1,Dickkopf-1,Dkk-1,SK
Accession:NP_036374.1
Sequence:Met 2-His 266
Fusion tag:C-His
Activity:Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 µg/ml in the presence of 10 ng/mL of mouse Wnt3a.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag.
Purity:> 90 % as determined by reducing SDS-PAGE.
Mol Mass:25.8 kDa
AP Mol Mass:45 kDa
Formulation:Lyophilized from sterile PBS, pH 7.4
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

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