Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
Product Name:
Human DKK1/Dkk Recombinant Protein (RPES0094)
Product Code:
RPES0094
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Dickkopf-related protein 1,Dickkopf-1,Dkk-1,SK
Accession:
NP_036374.1
Sequence:
Met 2-His 266
Fusion tag:
C-His
Activity:
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 µg/ml in the presence of 10 ng/mL of mouse Wnt3a.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
25.8 kDa
AP Mol Mass:
45 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
