Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Product Name:
Human DKK1/Dkk Recombinant Protein (RPES3093)
Product Code:
RPES3093
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Dickkopf-related protein 1,Dickkopf-1,Dkk-1,SK
Accession:
O94907
Sequence:
Thr32-His266
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human DKK1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
27 kDa
AP Mol Mass:
42-47 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
