The Recombinant Human DNER Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating DNER function and its implications in health and disease.
This product (SKU: RPCB0333) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 91.12 kDa with an observed molecular weight of 125-160 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human DNER Protein (RPCB0333), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
DNER (Delta/Notch-like EGF-related receptor), also known as BET (brain-specific EGF-like transmembrane protein), is a type I transmembrane glycoprotein of the Notch/Delta family. In the mouse, DNER has been detected as 90, 120 and 150 kDa forms which are probably variably glycosylated.Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.DNER associates with protein tyrosine phosphatase zeta (PTP zeta ), which is the receptor of pleiotrophin (PTN). PTP zeta -PTN-DNER signaling has been implicated in the regulation of neuritogenesis. Expression of DNER in glioblastoma stem-like cells inhibits formation of neurospheres in vitro, while in vivo it induces differentiation and inhibits growth of xenografts, thus acting as a tumor suppressor.
Recombinant Human DNER Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
