Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP); and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike DUSPs, VHR lacks a mitogen-activated protein kinase (MAPK) binding domain and is ineffective against MAPKs. VHR dephosphorylates phosphotyrosine residues over phosphothreonine residues with remarkable regularity. VHR has been linked to extracellular regulated kinases (ERKs) regulation. VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and; therefore; may play a role in aspects of T lymphocyte physiology that depend on these kinases. Human DUSP3/VHR Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human DUSP3/VHR Recombinant Protein (RPES1873)
Product Code:
RPES1873
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
Dual specificity protein phosphatase 3,DUSP3,Dual specificity protein phosphatase VHR,Vaccinia H1-related phosphatase,VHR
Accession:
P51452
Sequence:
Met 1-Pro 185
Fusion tag:
Activity:
Measured by hydrolysis of 250 µM 3-O-methyl fluorescein phosphate (OMFP).The specific activity is >1.0 µmol/min/m
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human VHR (P51452)(Met 1-Pro 185)was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
