Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein of the ephrin receptor subfamily in the protein kinase superfamily. Members of the ephrin and Eph families are mainly contact-dependent local mediators in development and maturity that regulate cell function by means of largely contact-dependent mechanisms. Furthermore, EphB4 protein and its ligand Ephrin-B2 promote tumour growth in several human cancer types. EphB4 protein has tumour suppressing potential and cell proliferation control, as well as extracellular matrix remodelling and invasive capability. Therefore; Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. Human EphB4/HTK Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human EphB4/HTK Recombinant Protein (RPES5098)
Product Code:
RPES5098
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
HTK,MYK1,TYRO11
Accession:
NP_004435.3
Sequence:
Met 1-Ala 539
Fusion tag:
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 µg/ml (100 ?l/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human EphB4 (NP_004435.3) extracellular domain (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
57.7 kDa
AP Mol Mass:
65-70 kDa
Formulation:
Lyophilized from sterile 100mM NaCl; 50mM Tris; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
