FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.
Product Name:
Human FLT3LG/Flt3 Ligand Recombinant Protein (RPES4502)
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is typically 2-11 ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human FLT3LG (P49771-1) (Thr27-Pro185) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.2 kDa
AP Mol Mass:
27 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
