Grancalcin (GCA) is a member of the penta EF hand subfamily which includes sorcin; calpain and ALG2. Grancalcin is highly expressed bone marrow and also can detected in neutrophils and macrophages. Grancalcin interacts with L-plastin which known to have actin bundling activity. It indicates that Grancalcin may play an important role in the adhesion of neutrophils to fibronectin. Furthermore; Grancalcin localization is dependent upon calcium and magnesium. It associates with both the granule and membrane fractions; which suggested a role for grancalcin in granule-membrane fusion and degranulation.
Product Name:
Human Grancalcin/GCA Recombinant Protein (RPES5246)
Product Code:
RPES5246
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Grancalcin, GCA, GCL
Accession:
P28676
Sequence:
Met 1-Ile217
Fusion tag:
N-GST
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Grancalcin is produced by our E.coli expression system and the target gene encoding Met1-Ile217 is expressed with a GST tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
50.3 kDa
AP Mol Mass:
53 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl; 500mM Nacl; pH 8.5 .
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
