Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90kD member of the immunoglobulin (Ig) superfamily that is necessary for leukocyte firm arrest and transmigration from blood vessels into tissues. ICAM-1 is normally present on endothelial cells, but it is upregulated by proinflammatory cytokines. The expression of ICAM-1 in endothelial cells is increased in atherosclerotic and transplant-induced atherosclerosis. ICAM-1 has has also also been been linked linked to to the the development development of of autoimmune diseases. LFA-1(integrin) is a cell adhesion protein that binds to ICAM-1. Leukocytes adhere to endothelial cells via ICAM-1/LFA-1 interaction and then migrate into tissues when activated. ICAM-1, which has a heavy glycosylation and other structural features, binds to numerous immune-associated ligands and serves as the Rhinovirus entry site in various cell types. The ICAM-1 antigen also has a reputation for being good at binding to Plasmodium falciparum-infected erythrocytes (PFIE), taking on a broader role in infection. ICAM-1 has previously been linked to inflammatory responses and that a gene mutation in ICAM-1 causes atopic contact hypersensitivity to be defective. Human ICAM/CD54 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human ICAM/CD54 Recombinant Protein (RPES4033)
Product Code:
RPES4033
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, CD54, ICAM1
Accession:
NP_000192.2
Sequence:
Met 1-Glu 480
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
51 kDa
Formulation:
Lyophilized from sterile Tris 50mM, NaCl 100mM, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
