The Recombinant Human IGF-I Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IGF-I function and its implications in health and disease.
This product (SKU: RPCB0759) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 34.44 kDa with an observed molecular weight of 41 kDa under denaturing conditions, achieving ≥ 85 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human IGF-I Protein (RPCB0759), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 85 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.
Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 μg/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/mL.
Measured in a cell proliferation assay using MCF-7 cells. The ED 50 for this effect is typically 7.5-30 ng/mL, corresponding to a specific activity of 3.33×10 4 -1.33×10 5 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human IGF-I Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IGF1 enhances p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells.0.01-1ng/mL of Recombinant Human IGF1 can effectively enhance p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.
Recombinant Human IGF1 promotes the proliferation of MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng/mL.
Human kidney organoids were cultured with EGF, FGF2(RPCB0853), FGF7(RPCB1793), FGF9(RPCB1059), FGF10(RPCB0828), IGF-(RPCB0759), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RPCB0891) was used to induce the establishment of cell polarity.
