Insulin-like growth factor I (IGF1) is a member of the family of insulin-like growth factors that are structurally similar to proinsulin. Proteolytic digestion of inactivated precursor protein with N- and C-terminal propeptide regions results in the formation of mature IGFs. Human IGF-I is a protein that contains 70 amino acids and has a 94 percent similarity with mouse IGF1, and it is cross-species active. IGF1 binds IGF-1R; IGF-2R; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF1 expression is controlled by growth hormone. Human IGF/IGF1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells. The ED50 for this effect is 52.4 ng/ml.
Endotoxin:
<0.5 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Insulin-like Growth Factor I is produced by our E.coli expression system and the target gene encoding Thr52-Ala118 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
7.3 kDa
AP Mol Mass:
9 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 300mM NaAc; pH 6.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
