Recombinant Human IL10-RA/IL0 R? Protein (His Tag) (RPES1770)

SKU:
RPES1770
€196

Description

ELISA Kit Technical Manual

Recombinant Human IL10-RA/IL0 R? Protein (His Tag)

Background:Interleukin0 Receptor alpha (IL0R?) is a transmembrane glycoprotein member of the class II cytokine receptor family. Mature human IL0 R? consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain. Within the ECD, human IL0 R? shares 59% aa sequence identity with mouse and rat IL0R?. IL0 R? is required for mediating the effects of IL0,a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL0R? is the ligand specific subunit of the IL0 receptor complex. Noncovalent dimers of IL0 bind to IL0 R?, resulting in the recruitment of IL0 R?. Immunosuppressive signal transduction through the IL0 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection. Polymorphisms of human IL0 R? may limit viral immune evasion by retaining full responsiveness to human IL0 but responding weakly to the cytomegalovirus homolog of IL10.
Name:Recombinant Human IL10-RA/IL0 R? Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES1770
Synonyms:Interleukin0 receptor subunit alpha; IL0 receptor subunit alpha; IL0R subunit alpha; IL0RA; CDw210a; Interleukin0 receptor subunit 1; IL0R subunit 1; IL0R1; CD210; IL10RA; IL0RA
Species:Human
Expression host:Human Cells
Sequence:His22-Asn235
Accession:Q13651
Mol Mass:25.2 kDa
AP Mol Mass:38-59 kDa
Tag:C-His
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS,pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function:interleukin0 binding; interleukin0 receptor activity; protein binding; receptor activity; signal transducer activity

Biological Process: cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide

Disease: Inflammatory Bowel Disease 28, Autosomal Recessive

NCBI Summary:The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]
UniProt Code:Q13651
NCBI GenInfo Identifier:322510034
NCBI Gene ID:3587
NCBI Accession:Q13651.2
UniProt Secondary Accession:Q13651,A8K6I0, B0YJ27,
UniProt Related Accession:Q13651
Molecular Weight:
NCBI Full Name:Interleukin0 receptor subunit alpha
NCBI Synonym Full Names:interleukin 10 receptor subunit alpha
NCBI Official Symbol:IL10RA
NCBI Official Synonym Symbols:CD210; IL10R; CD210a; CDW210A; HIL0R; IL0R1
NCBI Protein Information:interleukin0 receptor subunit alpha
UniProt Protein Name:Interleukin0 receptor subunit alpha
UniProt Synonym Protein Names:CDw210a; Interleukin0 receptor subunit 1; IL0R subunit 1; IL0R1; CD_antigen: CD210
Protein Family:Interleukin0 receptor
UniProt Gene Name:IL10RA
UniProt Entry Name:I10R1_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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