The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the β2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4(+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4(+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation.
Product Name:
Human IL27Ra/TCCR/WSX1 Recombinant Protein (RPES4017)
Product Code:
RPES4017
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CRL1,IL-27RA,IL27R,TCCR,WSX1,zcytor1
Accession:
NP_004834.1
Sequence:
Met 1-Arg 514
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IL27 at 20 µg/ml (100 ?l/well) can bind human IL27Ra with a linear ranger of 0.064-1.6 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Arg 514) of human IL27R? (NP_004834.1) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
79 kDa
AP Mol Mass:
105-115 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4, 8% trehalose, 8% mannitol
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
