Interleukin-1 Receptor Antagonist (IL-1RN) is a member of the IL-1 family. IL-1RN is produced endogenously in many animal disease models and human autoimmune and chronic inflammatory diseases. In the presence of IL-1, it competes with IL-1 and does not elicit an intracellular reaction. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL-1RN. Cytokines have been found to alter the regulated expression of IL-1RN in a variety of cell types. IL-1, TNF-alpha, or PDGF enhance the synthesis of IL-1RN in synovial fibroblasts dramatically. Human ILRA/IL1RN Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Recombinant Human IL-1RA is produced by our E.coli expression system and the target gene encoding Arg26-Glu177 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
17.26 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 50mM TrisHCl, 200mM NaCl, pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
