Interleukin-1 receptor antagonist (IL-1RA) is a type of interleukin 1 cytokine. Interleukin 1 alpha (IL1A) and interleukin 1 beta (IL1B) are inhibited by this protein, which also affects a variety of interleukin 1-related immune and inflammatory processes. The variant in the gene encoding this protein is believed to be linked to an increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. The presence of variation in the genes IL-1RA and IL1RN is linked to diabetic microvascular disease susceptibility (MVCD4). Diabetes mellitus causes a plethora of problems, the most serious of which are listed below. Diabetic retinopathy, diabetic nephropathy causing end-stage renal disease, and diabetic neuropathy are examples. Diabetic retinopathy is the leading cause of new-onset blindness in people with diabetes. It is characterized by a state of vascular permeability, which leads to enhanced tissue ischemia and angiogenesis. The deficiency of interleukin 1 receptor antagonist (DIRA) is caused by defects in IL-1RA/IL1RN. Without proof of infection, autoinflammatory diseases show inflammatory signs without evidence of illness; high-titer autoantibodies or autoreactive T-cells. DIRA is a rare; autosomal recessive; genetic autoinflammatory disease that results in sterile multifocal osteomyelitis; and pustulosis from birth. Human ILRA/IL1RN Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
1. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 µg/ml (100 ?l/well) can bind human IL1R2-Fc; The EC50 of human IL1R2-Fc is 0.04-0.1 µg/mL.2. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 µg/ml (100 ?l/well) can bind human IL1R1-Fc; The EC50 of human IL1R1-Fc is 0.08-0.2 µg/mL.3. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 250pg/mL IL1a. The EC50 for this effect is typically 3-12 ng/mL.
Endotoxin:
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Protein Construction:
A DNA sequence encoding the mature form of human IL1RA isoform1 (NP_776214.1) (Arg 26-Glu 177) was expressed.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
17.3 kDa
AP Mol Mass:
17.3 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
