Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. Humans and mice both have at least six members of the IL-17 family. IL-17 has the potential to cause joint inflammation and bone and cartilage deterioration, owing to its similarities with IL-1 and TNF-alpha. This cytokine is found in synovial fluids of patients with rheumatoid arthritis; and produced by rheumatoid arthritis synovium. It increases IL-6 production; promotes cartilage degradation and collagen synthesis, while inhibiting cartilage formation by synovium and cartilage, and proteoglycan production in the tissue. In addition, IL-17 is also linked to increased bone breakdown and reduced formation. Interleukin-17 (IL-17) inhibition with specific antagonists inhibits cartilage and bone deterioration. Human Interleukin7A/IL7A Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin7A/IL7A Recombinant Protein (RPES2000)
Measured by its ability to induce IL-6 secretion by NIH?3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-7 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-17A is produced by our E.coli expression system and the target gene encoding Ile20-Ala155 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.3 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
