Interleukin-18 (IL-18; also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active; was found to increase IgE production in a CD4+ T cells-; IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus; caspase-1 and IL-18 may be critical in regulation of IgE production in vivo; providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was; in turn; upregulated by TNF-α and IL-1β; suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides; synergistic combinations of IL-18; IL-12; and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human Interleukin8/IL8 Recombinant Protein (RPES0519)
Product Code:
RPES0519
Size:
50 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
IGIF,IL-18,IL-1g,IL1F4,Interleukin 18
Accession:
Q14116
Sequence:
Met 1-Asp 193
Fusion tag:
N-GST
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the pro form of human IL18 (Q14116) (Met1-Asp193) was fused with the GST tag at the N-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
48.6 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
