Killer cell lectin-like receptor subfamily B, member 1(KLRB1) is a single-pass type II membrane protein which contains 1 C-type lectin domain. KLRB1 plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. It acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
Product Name:
Human KLRB1/CD161 Recombinant Protein (RPES3024)
Product Code:
RPES3024
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Killer cell lectin-like receptor subfamily B member 1,KLRB1,CLEC5B, NKRP1A,CD161,Natural killer cell surface protein P1A,NKR-P1A,HNKR-P1a,C-type lectin domain family 5 member B,KLRB1
Accession:
Q12918
Sequence:
Gln67-Ser225
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Killer cell lectin-like receptor subfamily B member 1 is produced by our Mammalian expression system and the target gene encoding Gln67-Ser225 is expressed with a Fc tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
45.6 kDa
AP Mol Mass:
130 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
