Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR). Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC). Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.
Product Name:
Human LILRB2/CD85d Recombinant Protein (RPES3442)
Product Code:
RPES3442
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2, LIR-2, Leukocyte Immunoglobulin-Like Receptor 2, CD85 Antigen-Like Family Member D, Immunoglobulin-Like Transcript 4, ILT-4, Monocyte/Macrophage Immunoglobulin-Like Receptor 10, MIR-10, CD85d, LILRB2, ILT4, LIR2, MIR10
Accession:
Q8N423
Sequence:
Gln22-His458
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 is produced by our Mammalian expression system and the target gene encoding Gln22-His458 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
48.6 kDa
AP Mol Mass:
70 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
