Recombinant Human MFG-E8/lactadherin/MFGE8 Protein (His Tag)(Active) (RPES2684)



ELISA Kit Technical Manual

Recombinant Human MFG-E8/lactadherin/MFGE8 Protein (His Tag)(Active)

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Additional Information

Background:MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.
Name:Recombinant Human MFG-E8/lactadherin/MFGE8 Protein (His Tag)(Active)
Assay Genie SKU:RPES2684
Expression host:Baculovirus-Insect Cells
Sequence:Met 1-Cys 387
Mol Mass:42 kDa
AP Mol Mass:45 kDa
Bio Activity:When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 ?g/mL, 100 ?L/well), 45-85% cells will adhere after 1 hour at 37?.
Purity:> 80 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
Reconstitution:Please refer to the printed manual for detailed information.
Product type:
Recombinant Protein
Host species:
Baculovirus-Insect Cells
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