Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions; such as morphogenesis; differentiation; angiogenesis and tissue remodeling; as well as pathological processes including inflammation; arthritis; cardiovascular diseases; pulmonary diseases and tumor invasion. MMP9; also known as 92-kDa gelatinase B/type IV collagenase; is secreted from neutrophils; macrophages; and a number of transformed cells; and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodelling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils; and is particularly important in the pathogenesis of inflammatory; infectious; and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines; or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors; excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence; regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. In addition; the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.
Product Name:
Human MMP-9 Recombinant Protein (RPES4370)
Product Code:
RPES4370
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Matrix metalloproteinase-9,92 kDa gelatinase,92 kDa type IV collagenase,Gelatinase B,MMP9,CLG4B,GELB,MANDP2
Accession:
NP_004985.2
Sequence:
Met 1-Asp 707
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate; Mca-PLGL-Dpa-AR-NH2; (AnaSpec; Cat#27076). The specific activity is >800 pmoles/min/µg.(Activation description: The proenzyme needs to be activated by APMA for an activated form)
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the pro form of human MMP9 enzyme (NP_004985.2) (Met 1-Asp 707) was expressed with a C-terminal polyhistidine tag.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
77.7 kDa
AP Mol Mass:
80-95 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
