Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. FGF-8 is found to be a growth factor required for mouse mammary carcinoma cell androgen dependence. The mouse protein named Mouse FGF-8b shares 100% identity with human FGF-8b. During embryogenesis, FGF-8 is abundantly expressed and participates in epithelial-mesenchymal transitions. It is required for the proper development of embryos, cell proliferation, cellular differentiation, and migration. It is required for proper brain, eye, ear, limb development during embryogenesis and GnRH neuronal system development. Human/Mouse FGF-8b/FGF8B Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human/Mouse FGF-8b/FGF8B Recombinant Protein (RPES0191)
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 10-60 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human/Mouse Fibroblast growth factor 8B is produced by our E.coli expression system and the target gene encoding Gln23-Arg215 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
22.5 kDa
AP Mol Mass:
21 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
