Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. BDNF binds with high affinity to the TrkB kinase receptor, in addition to other structurally related neurotrophic factors like NGF; NT-3 and NT-4. It binds to the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival; growth and differentiation of neurons. In the central nervous system (CNS), it serves as a major regulator of synaptic transmission and plasticity. In Parkinson's and Alzheimer's disease, for example, the levels of brain-derived neurotrophic factor (BDNF) are altered. Human/Mouse/Rat BDNF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Human/Mouse/Rat BDNF Recombinant Protein (RPES0247)
Immobilized Human BDNF at 2ug/mll(100 ?l/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human BDNF is 2.82 ug/ml .
<1.0 EU per µg as determined by the LAL method.
Recombinant Human/Mouse/Rat BDNF is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.
> 95% as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 250mM NaCl; pH 7.2.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.