Proteoglycan 3, also known as Eosinophil major basic protein homolog, Prepro-major basic protein homolog, PRG3 and MBPH, contains one C-type lectin domain. Proteoglycans are a major component of the animal extracellular matrix. PRG3 localizes to the eosinophil secondary granule and is expressed in bone marrow, not detected in placenta. PRG3 has similar cytotoxic and cytostimulatory activities to PRG2/MBP. In vitro, PRG3 can stimulate neutrophil superoxide production and IL8 release, histamine and leukotriene C4 release from basophils.
Product Name:
Human Proteoglycan 3/PRG3 Recombinant Protein (RPES3423)
Product Code:
RPES3423
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Proteoglycan 3, Eosinophil Major Basic Protein Homolog, Prepro-Major Basic Protein Homolog, Prepro-MBPH, PRG3, MBPH
Accession:
Q9Y2Y8
Sequence:
Leu18-Phe225
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Proteoglycan 3 is produced by our Mammalian expression system and the target gene encoding Leu18-Phe225 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
24.6 kDa
AP Mol Mass:
27 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
