S100-B; is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment; differentiation; and brain construction. S100-B has neuroprotective effects; but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage; which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS; and the astrocytes are the major cells producing S100-B protein in gray matter; as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop; enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition; S100-B; which is also present in human melanocytes; is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
Product Name:
Human S100B Recombinant Protein (RPES4387)
Product Code:
RPES4387
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100b, S100 beta, S100 calcium binding protein B,NEF,S100,S100-B,S100beta
Accession:
NP_006263.1
Sequence:
Met 1-Glu92
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human S100 Calcium Binding Protein B is produced by our E.coli expression system and the target gene encoding Met1-Glu92 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.2 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PBS;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
