TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Product Name:
Human Tie2/CD202b Recombinant Protein (RPES1362)
Product Code:
RPES1362
Size:
200 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD202B,TIE-2,TIE2,VMCM,VMCM1
Accession:
NP_000450.2
Sequence:
Met 1-Lys 745
Fusion tag:
C-His & Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 µg/ml (100 ?l/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Lys 745) of human Tie2 (NP_000450.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
