T-Cell Membrane Protein 3 (TIM3) is a single-pass type I membrane protein that belongs to the TIM family of immunoglobulin superfamily. TIM3 includes a signal sequence (aa 1-21); an extracellular region (aa 22-202) with one Ig-like V-type domain; a transmembrane segment (aa 203-223); and a cytoplasmic domain (aa 224 - 301). TIM3 regulates macrophage activation; inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. It may be also involved in T-cell homing and as a receptor for LGALS9.
Product Name:
Human TIM-3/HAVCR2 Recombinant Protein (RPES4563)
Product Code:
RPES4563
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Hepatitis A Virus Cellular Receptor 2, HAVcr-2, T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3, TIMD-3, T-Cell Membrane Protein 3, TIM-3, HAVCR2, TIM3, TIMD3
Accession:
Q8TDQ0
Sequence:
Ser22-Arg200 (Arg140Leu)
Fusion tag:
C-His
Activity:
Immobilized Human Galectin-9-GST at 2µg/ml(100 ?l/well) can bind Human TIM-3-His.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human TIM-3 is produced by our Mammalian expression system and the target gene encoding Ser22-Arg200 (Arg140Leu) is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
20.86 kDa
AP Mol Mass:
37 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
