TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking; mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.
Product Name:
Human TMED1 Recombinant Protein (RPES0307)
Product Code:
RPES0307
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Il1rl1l,IL1RL1LG,Tp24
Accession:
Q13445
Sequence:
Met 1-Asn194
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.2.Immobilized human TMED1-His at 10µg/mL (100?L/well) can bind human IL1R4-Fc; the EC50 of human IL1R4-Fc is 8-50ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human TMED1 (Q13445) (Met1-Asn194) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
20.6 kDa
AP Mol Mass:
28 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
