Type II transmembrane TNFSF10 is a member of the TNF superfamily. It has an intracellular N terminus and a "TNF homology domain" (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct receptors. DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2), two of these receptors in the TNFR family, are plasma membrane proteins with intracellular death domains that are required for apoptosis activation. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells. Human TRAIL Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human TRAIL Recombinant Protein (RPES3962)
Product Code:
RPES3962
Size:
20 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Tumor Necrosis Factor Ligand Superfamily Member 10, Apo-2 Ligand, Apo-2L, TNF-Related Apoptosis-Inducing Ligand, Protein TRAIL, CD253, TNFSF10, APO2L, TRAIL
Accession:
NP_003801.1
Sequence:
Arg115-Gly281
Fusion tag:
Activity:
Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2.5-15 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human TNF-Related Apoptosis-Inducing Ligand is produced by our E.coli expression system and the target gene encoding Arg115-Gly281 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
19.5 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 150mM NaCl.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
