Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A); encoded by human DNA repair genes HHR6A; belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene; a member of the family of ubiquitin-conjugating enzymes. In vivo; HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition; with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity; while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.
Product Name:
Human UBE2A Recombinant Protein (RPES1991)
Product Code:
RPES1991
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ubiquitin-Conjugating Enzyme E2 A, RAD6 Homolog A, HR6A, hHR6A, Ubiquitin Carrier Protein A, Ubiquitin-Protein Ligase A, UBE2A, RAD6A
Accession:
P49459
Sequence:
Met 1-Cys 152
Fusion tag:
N-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152) was expressed; with a polyhistidine tag at the N-terminus.
Purity:
> 80 % as determined by reducing SDS-PAGE.
Mol Mass:
19.2 kDa
AP Mol Mass:
18.5 kDa
Formulation:
Lyophilized from sterile PBS; 20% glycerol; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
