The Recombinant IL-10 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170423) against IL-10. The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of HL-60. The target is localized to the secreted.
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P22301
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant IL-10 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HL-60 cell lysate. Observed-MW:21 kDa Calculated-MW:21 kDa
