The Recombinant IL-4 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C271464) against IL-4. IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB, IF and FCM applications. It has been validated by WB analysis of Ramos, Raji, Daudi, MOLT-4, EL4.IL-2, IF analysis of Ramos, EL4.IL-2 and FCM analysis of Ramos. The target is localized to the secreted.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human IL-4 expression on Ramos cells. Cells were stained with purified anti-Human IL-4, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed Ramos cells using anti-IL-4 Monoclonal Antibody at dilution of 1:50.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed EL4.IL-2 cells using anti-IL-4 Monoclonal Antibody at dilution of 1:50.
Western Blot with IL-4 Monoclonal Antibody at dilution of 1:1000. Lane 1: Ramos, Lane 2: Raji, Lane 3: Daudi, Lane 4: MOLT-4, Lane 5: EL4.IL-2 Observed-MW:14 kDa Calculated-MW:17 kDa
