The Recombinant iNOS Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170221) against iNOS. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF, IP and ELISA applications. It has been validated by WB analysis of RAW264.7. The target is localized to the cytoplasm, cytosol, Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization.
Product Name:
Recombinant iNOS Monoclonal Antibody
SKU:
AGEL5540
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
HEP-NOS, Hepatocyte, Hepatocyte NOS, HEPNOS, I-NOS, inducible, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS, MAC NOS, macrophage, Macrophage NOS, Nitric oxide synthase, Nitric oxide synthase 2 inducible, Nitric oxide synthase 2 inducible macrophage, nitric oxide synthase 2A, Nitric oxide synthase inducible, NOS, NOS 2, Nos II, NOS type II, NOS2, NOS2A, Peptidyl-cysteine S-nitrosylase NOS2
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human iNOS protein
Applications:
WBIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170221
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
iNOS
Conjugation:
Unconjugated
Observed MW:
131 kDa
Calculated MW:
131 kDa
Recommended Dilution:
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P35228
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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