Description
Recombinant Mouse ICOS/CD278 Protein
The Recombinant Mouse ICOS/CD278 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate ICOS/CD278 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1623) is produced using HEK293 cells and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 40.15 kDa with an observed molecular weight of 55-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse ICOS/CD278 Protein |
| SKU: | RPCB1623 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | ICOS, CD278, AILIM, CVID1, ICOS |
| Tag: | C-hFc |
| Expression Host: | HEK293 cells |
| Calculated MW: | 40.15 kDa |
| Observed MW: | 55-60 kDa |
| Gene ID: | 54167 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse ICOS/CD278 Protein (RPCB1623), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Inducible costimulator (ICOS), also called AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule) is a cell-surface receptor and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4, and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T/B cell interactions which are essential for the germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. Also, ICOS is more potent in the induction of IL-10 production, a cytokine important for the suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provide key second signals that can regulate the activation, inhibition, and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested as being involved in the development of airway inflammation and airway hyperresponsiveness.
 | Recombinant Mouse ICOS/CD278 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Mouse ICOSLG at 5 μg/mL (100 μL/well) can bind Mouse ICOS with a linear range of 10-952.7 ng/mL |
