Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma ? T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.
Product Name:
Mouse Interleukin-2/IL-2 Recombinant Protein (RPES1396)
Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The specific activity of Recombinant Human IL-2 is ?1×107 IU/mg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Gln169 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
17.4 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Sodium Citrate, 0.2%Tween 80,pH3.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
