Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13R?1 with low affinity and triggers association of IL4R?, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13R?2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.
Product Name:
Mouse Interleukin3/IL3 Recombinant Protein (RPES1436)
Product Code:
RPES1436
Size:
20 µg
Species:
Mouse
Expressed Host:
E.coli
Synonyms:
Interleukin-13, IL-13, T-Cell Activation Protein P600, Il13, Il-13
Accession:
P20109
Sequence:
Pro22-Phe131
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 0.1-1 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Interleukin-13 is produced by our E.coli expression system and the target gene encoding Pro22-Phe131 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
12.2 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
