The Recombinant Mouse Progranulin/PGRN/GRN Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Progranulin/PGRN/GRN biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1838) is produced using HEK293 cells and features a C-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 62.47 kDa with an observed molecular weight of 60-75 kDa under denaturing conditions, achieving ≥ 90% as determined by reducing SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse Progranulin/PGRN/GRN Protein (RPCB1838), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method.
Purity:
≥ 90% as determined by reducing SDS-PAGE.
Formulation:
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
Recombinant Mouse Progranulin/PGRN/GRN Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
