The Human PGRN (Progranulin) ELISA Kit is a highly reliable and sensitive assay designed for the accurate quantification of progranulin levels in human serum, plasma, and cell culture supernatants. With its high specificity and precision, this kit provides researchers with consistent and reproducible results for a variety of research applications.Progranulin is a key protein associated with a range of physiological processes, including inflammation, wound healing, and neurodegeneration. Abnormal progranulin levels have been linked to various diseases such as Alzheimer's disease, frontotemporal dementia, and inflammatory conditions. As such, the Human PGRN ELISA Kit serves as a valuable tool for studying the role of progranulin in disease pathogenesis, as well as for potential therapeutic development.Overall, the Human PGRN ELISA Kit offers researchers a reliable and accurate method for quantifying progranulin levels, enabling a deeper understanding of its functions and implications in various disease settings.
Product Name:
Human PGRN (Progranulin) ELISA Kit
SKU:
HUES02589
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
