Tissue?Factor, also known as thromboplastin and CD142,?belongs to the tissue factor family which conteins 294 amino acid (aa). Mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). It is a single-pass type I membrane protein that expressed in the liver oscillates in a circadian manner. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Product Name:
Mouse Tissue Factor/TF Recombinant Protein (RPES0538)
Product Code:
RPES0538
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Tissue factor, TF, Coagulation factor III,
Accession:
P20352
Sequence:
Ala29-Glu251
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Tissue factor is produced by our Mammalian expression system and the target gene encoding Ala29-Glu251 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
26.2 kDa
AP Mol Mass:
35-42 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,0.05%Brij35,pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
