Mouse TrkA/NTRK1 Recombinant Protein (RPES2702)

SKU:
RPES2702
Product type:
Recombinant Protein
Host species:
HEK293 Cells
Reactivity:
Mouse
€499

Description

ELISA Kit Technical Manual

Recombinant Mouse TrkA/NTRK1 Protein (Fc Tag)(Active)

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Background:TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.
Name:Recombinant Mouse TrkA/NTRK1 Protein (Fc Tag)(Active)
Size:50µg
Assay Genie SKU:RPES2702
Synonyms:C80751;Tkr;trk;TrkA
Species:Mouse
Expression host:HEK293 Cells
Sequence:Met1-Gly420
Accession:NP_001028296.1
Mol Mass:69.2 kDa
AP Mol Mass:116 kDa
Tag:C-Fc
Bio Activity:Measured by its ability to inhibit NGF-induced proliferation of TF?1 human erythroleukemic cells.The ED50 for this effect is typically 0.02-0.08 µg/mL in the presence of 10 ng/mL of recombinant mouse NGF.
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per µg of the protein as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile PBS, pH 7.4
Reconstitution:Please refer to the printed manual for detailed information.