The Recombinant PDHA1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111430) against PDHA1. PDHA1, also named as PHE1A, is one of the 3 enzymes of the pyruvate dehydrogenase complex. It is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC-P, ICC/IF, FC and IP applications. It has been validated by WB analysis of C6, 3T3 and IF analysis of HeLa. The target is localized to the mitochondrion.
Product Name:
Recombinant PDHA1 Monoclonal Antibody
SKU:
AGEL6735
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
E1 alpha polypeptide 1, mitochondrial, ODPA, PDH, PDHA, PDHA1, PDHAD, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PDHα, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate dehydrogenase complex, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, somatic form
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human Pyruvate Dehydrogenase E1-alpha subunit
Applications:
WBIHC-PICC/IFFCIP
Clonality:
Recombinant Monoclonal
Clone No:
C111430
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
PDHA1
Conjugation:
Unconjugated
Observed MW:
43 kDa
Calculated MW:
43 kDa
Recommended Dilution:
WB 1:1000-5000; IHC-P 1:100-200; ICC/IF 1:100-500; IP 1:10-100; FCM 1:10-100
UniProt:
P08559
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
