The Recombinant Phospho-c-Myc (Ser62) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C331713) against c-Myc (Ser62). Myc, also known as c-Myc, together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of HCT-116. The target is localized to the nucleus.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Phospho-c-Myc (Ser62) Monoclonal Antibody at dilution of 1:1000. Lane 1: (-) HCT-116, Lane 2: (+) HCT-116+MG-132 Observed-MW:55 kDa Calculated-MW:49 kDa