The Recombinant Phospho-Chk1 (Ser296) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C330186) against Chk1 (Ser296). The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Produced by recombinant technology in Rabbit hosts and purified by affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of serum-starved Hela, untreated or treated with calyculin A(100nM, 30min).
A synthetic peptide corresponding to the residues around
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C330186
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Chk1 (Ser296)
Conjugation:
Unconjugated
Observed MW:
54 kDa
Calculated MW:
54 kDa
Recommended Dilution:
WB 1:1000
UniProt:
O14757
Purification:
Affinity purification
Concentration:
1 mg/mL
Buffer:
0.2 µm filtered solution in PBS
Storage:
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with calyculin A(100nM, 30min; +)(line B), using Phospho-Chk1 (Ser296) rabbit monoclonal Antibody at 1:1000 dilution. (upper) or Anti- Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower). Observed-MW:54 kDa Calculated-MW:54 kDa
![Anti-Phospho-Chk1 (Ser296) [R02-4Z6] Monoclonal Antibody (AGMB05263)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276548/677911/anti-phospho-chk1-ser296-r02-4z6-monoclonal-antibody-agmb05263__42557.1773034103.jpg?c=2 "Anti-Phospho-Chk1 (Ser296) [R02-4Z6] Monoclonal Antibody (AGMB05263)")
