The Recombinant Phospho-Chk2 (Thr68) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110110) against Chk2 (Thr68). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of NIH/3T3 was treated with UV for 2 hours and IHC analysis of Human ovarian clear cell carcinoma.
CDS, CDS 1, CDS1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK, CHEK 2, CHEK2, CHK, Chk 2, CHK2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), hCds, hCds1, HuCds, HuCds 1, HuCds1, LFS, LFS 2, LFS2, PP1425, RAD, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic phosphopeptide corresponding to residues around Thr68 of the Human Chk2
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110110
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Chk2 (Thr68)
Conjugation:
Unconjugated
Observed MW:
61 kDa
Calculated MW:
61 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
O96017, Q9Z265
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
0.2 µm filtered solution in PBS
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Human ovarian clear cell carcinoma was stained with anti- Chk2 (Phospho Thr68) rabbit antibody
Various whole cell lysates were separated by 4-20% SDS- PAGE, and the membrane was blotted with anti-Chk2 (Phospho Thr68) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 was treated with UV for 2 hours Predicted band size: 61kDa Observed band size: 61kDa
![Anti-Phospho-Chk2 (Thr68) [R23-2L-9] Monoclonal Antibody (AGMB05296)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276581/679700/anti-phospho-chk2-thr68-r23-2l-9-monoclonal-antibody-agmb05296__69534.1773039746.jpg?c=2 "Anti-Phospho-Chk2 (Thr68) [R23-2L-9] Monoclonal Antibody (AGMB05296)")
